Journal articles 2015
Documents
No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums
Leiser WL, Olatoye MO, Rattunde HFW, Neumann G, Weltzien E and Haussmann BIG (2015). No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums. Plant and Soil Published online: 14 March 2015 (DOI: 10.1007/s11104-015-2437-1). Not open access; view abstract. (G7010.03.03)
Leiser WL, Olatoye MO, Rattunde HFW, Neumann G, Weltzien E and Haussmann BIG (2015). No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums. Plant and Soil Published online: 14 March 2015 (DOI: 10.1007/s11104-015-2437-1). Not open access; view abstract. (G7010.03.03)
Development of a SNP genotyping panel for detecting polymorphisms in Oryza glaberrima/O. sativa interspecific crosses
Pariasca-Tanaka J, Lorieux M, He C, McCouch S, Thomson MJ and Wissuwa M (2015). Development of a SNP genotyping panel for detecting polymorphisms in Oryza glaberrima/O. sativa interspecific crosses. Euphytica 201(1):67–78 (DOI: 10.1007/s10681-014-1183-4). First published in June 2014. Not open access; view abstract. (G3005.10)
Pariasca-Tanaka J, Lorieux M, He C, McCouch S, Thomson MJ and Wissuwa M (2015). Development of a SNP genotyping panel for detecting polymorphisms in Oryza glaberrima/O. sativa interspecific crosses. Euphytica 201(1):67–78 (DOI: 10.1007/s10681-014-1183-4). First published in June 2014. Not open access; view abstract. (G3005.10)
Novel QTLs in an interspecific backcross Oryza sativa x Oryza glaberrima for resistance to iron toxicity in rice
Dufey I, Draye X, Lutts S, Lorieux M, Martinez C and Bertin P (2015). Novel QTLs in an interspecific backcross Oryza sativa x Oryza glaberrima for resistance to iron toxicity in rice. Euphytica Published online: 1 February 2015 (DOI 10.1007/s10681-014-1342-7). Not open access; view abstract. (G3005.10)
Dufey I, Draye X, Lutts S, Lorieux M, Martinez C and Bertin P (2015). Novel QTLs in an interspecific backcross Oryza sativa x Oryza glaberrima for resistance to iron toxicity in rice. Euphytica Published online: 1 February 2015 (DOI 10.1007/s10681-014-1342-7). Not open access; view abstract. (G3005.10)
Registration of the Ki14 × B73 recombinant inbred mapping population of maize
Pratt RC, Holland JB, Balint-Kurti PJ, Coles ND, Zwonitzer JC, Casey MA and McMullens MC (2015). Registration of the Ki14 × B73 recombinant inbred mapping population of maize. Journal of Plant Registrations 9(2):262–265 (DOI: 10.3198/jpr2014.06.0041crmp). Not open access; view abstract.
Pratt RC, Holland JB, Balint-Kurti PJ, Coles ND, Zwonitzer JC, Casey MA and McMullens MC (2015). Registration of the Ki14 × B73 recombinant inbred mapping population of maize. Journal of Plant Registrations 9(2):262–265 (DOI: 10.3198/jpr2014.06.0041crmp). Not open access; view abstract.
Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress
Wang SG, Jia SS, Sun DZ, Wang HY, Dong FF, Ma HX, Jing RL and Ma G (2015). Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress. Photosynthetica 53(2):299–305 (DOI: 10.1007/s11099-015-0114-5). Not open access; view abstract. (G7010.02.01)
Wang SG, Jia SS, Sun DZ, Wang HY, Dong FF, Ma HX, Jing RL and Ma G (2015). Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress. Photosynthetica 53(2):299–305 (DOI: 10.1007/s11099-015-0114-5). Not open access; view abstract. (G7010.02.01)
Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat
Azam F, Chang X and Jing R (2015). Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat. Euphytica 202(2):245–258 (DOI: 10.1007/s10681-014-1283-1). First published online in October 2014. (G7010.02.01)
Abstract: High temperature or heat stress is one of the most important abiotic stresses that affect wheat production in almost every part of the world. Parameters of chlorophyll fluorescence kinetics (PCFKs) are the most powerful and reliable characters available to understand the impact of various abiotic stresses on plant physiological processes and heat tolerance. The present research was aimed to identify genomic regions controlling PCFKs at early growth stages of wheat through quantitative trait loci analysis by applying heat stress for different duration of time. A doubled haploid population derived from the cross of two Chinese wheat cultivars Hanxuan 10 and Lumai 14 was exposed to 38 °C for 2, 4, 6 and 8 h of heat stress and PCFKs (initial fluorescence, maximum fluorescence, variable fluorescence and maximum quantum efficiency of photosystem II) were measured. A total of 37 QTLs were identified for the target traits, among which 13 were detected under normal temperature of 25 °C and the remaining 24 under the stressful temperature of 38 °C. Stable or consistently expressed QTLs for initial, maximum and variable fluorescence were detected on chromosomes 1A, 1B, 2B, 4A and 7D. In addition, 24 QTLs were clustered in 9 clusters on chromosomes 1A, 1B, 2B, 3B, 3D, 4A, 5A and 7D. These QTL hot spot regions along with stable QTLs should be targeted for better understanding the genetic basis of chlorophyll fluorescence kinetics parameters in future mapping studies.
Azam F, Chang X and Jing R (2015). Mapping QTL for chlorophyll fluorescence kinetics parameters at seedling stage as indicators of heat tolerance in wheat. Euphytica 202(2):245–258 (DOI: 10.1007/s10681-014-1283-1). First published online in October 2014. (G7010.02.01)
Abstract: High temperature or heat stress is one of the most important abiotic stresses that affect wheat production in almost every part of the world. Parameters of chlorophyll fluorescence kinetics (PCFKs) are the most powerful and reliable characters available to understand the impact of various abiotic stresses on plant physiological processes and heat tolerance. The present research was aimed to identify genomic regions controlling PCFKs at early growth stages of wheat through quantitative trait loci analysis by applying heat stress for different duration of time. A doubled haploid population derived from the cross of two Chinese wheat cultivars Hanxuan 10 and Lumai 14 was exposed to 38 °C for 2, 4, 6 and 8 h of heat stress and PCFKs (initial fluorescence, maximum fluorescence, variable fluorescence and maximum quantum efficiency of photosystem II) were measured. A total of 37 QTLs were identified for the target traits, among which 13 were detected under normal temperature of 25 °C and the remaining 24 under the stressful temperature of 38 °C. Stable or consistently expressed QTLs for initial, maximum and variable fluorescence were detected on chromosomes 1A, 1B, 2B, 4A and 7D. In addition, 24 QTLs were clustered in 9 clusters on chromosomes 1A, 1B, 2B, 3B, 3D, 4A, 5A and 7D. These QTL hot spot regions along with stable QTLs should be targeted for better understanding the genetic basis of chlorophyll fluorescence kinetics parameters in future mapping studies.
Full article
Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.)
Keller B, Manzanares C, Jara C, Lobaton JD, Studer B and Raatz B (2015). Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 128(5):813–826 (DOI: 10.1007/s00122-015-2472-6). (G6010.03)
Key message: A major QTL for angular leaf spot resistance in the common bean accession G5686 was fine-mapped to a region containing 36 candidate genes. Markers have been developed for marker-assisted selection.
Abstract: Common bean (Phaseolus vulgaris L.) is an important grain legume and an essential protein source for human nutrition in developing countries. Angular leaf spot (ALS) caused by the pathogen Pseudocercospora griseola (Sacc.) Crous and U. Braun is responsible for severe yield losses of up to 80 %. Breeding for resistant cultivars is the most ecological and economical means to control ALS and is particularly important for yield stability in low-input agriculture. Here, we report on a fine-mapping approach of a major quantitative trait locus (QTL) ALS4.1GS, UC for ALS resistance in a mapping population derived from the resistant genotype G5686 and the susceptible cultivar Sprite. 180 F3 individuals of the mapping population were evaluated for ALS resistance and genotyped with 22 markers distributed over 11 genome regions colocating with previously reported QTL for ALS resistance. Multiple QTL analysis identified three QTL regions, including one major QTL on chromosome Pv04 at 43.7 Mbp explaining over 75 % of the observed variation for ALS resistance. Additional evaluation of 153 F4, 89 BC1F2 and 139 F4/F5/BC1F3 descendants with markers in the region of the major QTL delimited the region to 418 kbp harboring 36 candidate genes. Among these, 11 serine/threonine protein kinases arranged in a repetitive array constitute promising candidate genes for controlling ALS resistance. Single nucleotide polymorphism markers cosegregating with the major QTL for ALS resistance have been developed and constitute the basis for marker-assisted introgression of ALS resistance into advanced breeding germplasm of common bean.
Keller B, Manzanares C, Jara C, Lobaton JD, Studer B and Raatz B (2015). Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 128(5):813–826 (DOI: 10.1007/s00122-015-2472-6). (G6010.03)
Key message: A major QTL for angular leaf spot resistance in the common bean accession G5686 was fine-mapped to a region containing 36 candidate genes. Markers have been developed for marker-assisted selection.
Abstract: Common bean (Phaseolus vulgaris L.) is an important grain legume and an essential protein source for human nutrition in developing countries. Angular leaf spot (ALS) caused by the pathogen Pseudocercospora griseola (Sacc.) Crous and U. Braun is responsible for severe yield losses of up to 80 %. Breeding for resistant cultivars is the most ecological and economical means to control ALS and is particularly important for yield stability in low-input agriculture. Here, we report on a fine-mapping approach of a major quantitative trait locus (QTL) ALS4.1GS, UC for ALS resistance in a mapping population derived from the resistant genotype G5686 and the susceptible cultivar Sprite. 180 F3 individuals of the mapping population were evaluated for ALS resistance and genotyped with 22 markers distributed over 11 genome regions colocating with previously reported QTL for ALS resistance. Multiple QTL analysis identified three QTL regions, including one major QTL on chromosome Pv04 at 43.7 Mbp explaining over 75 % of the observed variation for ALS resistance. Additional evaluation of 153 F4, 89 BC1F2 and 139 F4/F5/BC1F3 descendants with markers in the region of the major QTL delimited the region to 418 kbp harboring 36 candidate genes. Among these, 11 serine/threonine protein kinases arranged in a repetitive array constitute promising candidate genes for controlling ALS resistance. Single nucleotide polymorphism markers cosegregating with the major QTL for ALS resistance have been developed and constitute the basis for marker-assisted introgression of ALS resistance into advanced breeding germplasm of common bean.
Abiotic stress responses in legumes: Strategies used to cope with environmental challenges
Araújo SS, Beebe S, Crespi M, Delbreil B, González EM, Gruber V, Lejeune-Henaut I, Link W, Monteros MJ, Prats E, Rao I, Vadez V and Vaz Patto MC (2015). Abiotic stress responses in legumes: Strategies used to cope with environmental challenges. Critical Reviews in Plant Sciences 34(1–3):237–280 (DOI: 10.1080/07352689.2014.898450). First published online in October 2014. Not open access; view abstract.
Araújo SS, Beebe S, Crespi M, Delbreil B, González EM, Gruber V, Lejeune-Henaut I, Link W, Monteros MJ, Prats E, Rao I, Vadez V and Vaz Patto MC (2015). Abiotic stress responses in legumes: Strategies used to cope with environmental challenges. Critical Reviews in Plant Sciences 34(1–3):237–280 (DOI: 10.1080/07352689.2014.898450). First published online in October 2014. Not open access; view abstract.
Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis
Leal-Bertioli S, Shirasawa K, Abernathy B, Moretzsohn M, Chavarro C, Clevenger J, Ozias-Akins P, Jackson S and Bertioli D (2015). Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis. Genetics 199(4):1093–1105 (DOI: 10.1534/genetics.115.174607). (G6010.01)
Abstract: Arachis hypogaea L. (cultivated peanut) is an allotetraploid (2n = 4x = 40) with an AABB genome type. Based on cytogenetic studies it has been assumed that peanut and wild-derived induced AABB allotetraploids have classic allotetraploid genetic behavior with diploid-like disomic recombination only between homologous chromosomes, at the exclusion of recombination between homeologous chromosomes. Using this assumption, numerous linkage map and quantitative trait loci studies have been carried out. Here, with a systematic analysis of genotyping and gene expression data, we show that this assumption is not entirely valid. In fact, autotetraploid-like tetrasomic recombination is surprisingly frequent in recombinant inbred lines generated from a cross of cultivated peanut and an induced allotetraploid derived from peanut's most probable ancestral species. We suggest that a better, more predictive genetic model for peanut is that of a "segmental allotetraploid" with partly disomic, partly tetrasomic genetic behavior. This intermediate genetic behavior has probably had a previously overseen, but significant, impact on the genome and genetics of cultivated peanut.
Leal-Bertioli S, Shirasawa K, Abernathy B, Moretzsohn M, Chavarro C, Clevenger J, Ozias-Akins P, Jackson S and Bertioli D (2015). Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis. Genetics 199(4):1093–1105 (DOI: 10.1534/genetics.115.174607). (G6010.01)
Abstract: Arachis hypogaea L. (cultivated peanut) is an allotetraploid (2n = 4x = 40) with an AABB genome type. Based on cytogenetic studies it has been assumed that peanut and wild-derived induced AABB allotetraploids have classic allotetraploid genetic behavior with diploid-like disomic recombination only between homologous chromosomes, at the exclusion of recombination between homeologous chromosomes. Using this assumption, numerous linkage map and quantitative trait loci studies have been carried out. Here, with a systematic analysis of genotyping and gene expression data, we show that this assumption is not entirely valid. In fact, autotetraploid-like tetrasomic recombination is surprisingly frequent in recombinant inbred lines generated from a cross of cultivated peanut and an induced allotetraploid derived from peanut's most probable ancestral species. We suggest that a better, more predictive genetic model for peanut is that of a "segmental allotetraploid" with partly disomic, partly tetrasomic genetic behavior. This intermediate genetic behavior has probably had a previously overseen, but significant, impact on the genome and genetics of cultivated peanut.
Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids
Leal-Bertioli SCM, Santos SP, Dantas KM, Inglis PW, Nielen S, Araujo ACG, Silva JP, Cavalcante U, Guimarães PM, Brasileiro ACM, Carrasquilla-Garcia N, Penmetsa RV, Cook D, Moretzsohn MC and Bertioli DJ (2015). Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids. Annals of Botany 115(2):237–49 (DOI: 10.1093/aob/mcu237). First published online in December 2014. (G6010.01)
Abstract: Background and Aims Arachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with an AABB genome. Arachis batizocoi was once considered the ancestral donor of the peanut B genome, but cytogenetics and DNA phylogenies have indicated a new genome classification, ‘K’. These observations seem inconsistent with genetic studies and breeding that have shown that A. batizocoi can behave as a B genome.
Methods The genetic behaviour, genome composition and phylogenetic position of A. batizocoi were studied using controlled hybridizations, induced tetraploidy, whole-genome in situ fluorescent hybridization (GISH) and molecular phylogenetics.
Key Results Sterile diploid hybrids containing AK genomes were obtained using A. batizocoi and the A genome species A. duranensis, A. stenosperma, A. correntina or A. villosa. From these, three types of AAKK allotetraploids were obtained, each in multiple independent polyploidy events. Induced allotetraploids were vigorous and fertile, and were hybridized to A. hypogaea to produce F1 hybrids. Even with the same parental combination, fertility of these F1 hybrids varied greatly, suggesting the influence of stochastic genetic or epigenetic events. Interestingly, hybrids with A. hypogaea ssp. hypogaea were significantly more fertile than those with the subspecies fastigiata. GISH in cultivated × induced allotetraploids hybrids (harbouring AABK genomes) and a molecular phylogeny using 16 intron sequences showed that the K genome is distinct, but more closely related to the B than to the A genome.
Conclusions The K genome of A. batizocoi is more related to B than to the A genome, but is distinct. As such, when incorporated in an induced allotetraploid (AAKK) it can behave as a B genome in crosses with peanut. However, the fertility of hybrids and their progeny depends upon the compatibility of the A genome interactions. The genetic distinctness of A. batizocoi makes it an important source of allelic diversity in itself, especially in crosses involving A. hypogaea ssp. hypogaea.
Leal-Bertioli SCM, Santos SP, Dantas KM, Inglis PW, Nielen S, Araujo ACG, Silva JP, Cavalcante U, Guimarães PM, Brasileiro ACM, Carrasquilla-Garcia N, Penmetsa RV, Cook D, Moretzsohn MC and Bertioli DJ (2015). Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids. Annals of Botany 115(2):237–49 (DOI: 10.1093/aob/mcu237). First published online in December 2014. (G6010.01)
Abstract: Background and Aims Arachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with an AABB genome. Arachis batizocoi was once considered the ancestral donor of the peanut B genome, but cytogenetics and DNA phylogenies have indicated a new genome classification, ‘K’. These observations seem inconsistent with genetic studies and breeding that have shown that A. batizocoi can behave as a B genome.
Methods The genetic behaviour, genome composition and phylogenetic position of A. batizocoi were studied using controlled hybridizations, induced tetraploidy, whole-genome in situ fluorescent hybridization (GISH) and molecular phylogenetics.
Key Results Sterile diploid hybrids containing AK genomes were obtained using A. batizocoi and the A genome species A. duranensis, A. stenosperma, A. correntina or A. villosa. From these, three types of AAKK allotetraploids were obtained, each in multiple independent polyploidy events. Induced allotetraploids were vigorous and fertile, and were hybridized to A. hypogaea to produce F1 hybrids. Even with the same parental combination, fertility of these F1 hybrids varied greatly, suggesting the influence of stochastic genetic or epigenetic events. Interestingly, hybrids with A. hypogaea ssp. hypogaea were significantly more fertile than those with the subspecies fastigiata. GISH in cultivated × induced allotetraploids hybrids (harbouring AABK genomes) and a molecular phylogeny using 16 intron sequences showed that the K genome is distinct, but more closely related to the B than to the A genome.
Conclusions The K genome of A. batizocoi is more related to B than to the A genome, but is distinct. As such, when incorporated in an induced allotetraploid (AAKK) it can behave as a B genome in crosses with peanut. However, the fertility of hybrids and their progeny depends upon the compatibility of the A genome interactions. The genetic distinctness of A. batizocoi makes it an important source of allelic diversity in itself, especially in crosses involving A. hypogaea ssp. hypogaea.
