Journal articles 2015
Documents
No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums
Leiser WL, Olatoye MO, Rattunde HFW, Neumann G, Weltzien E and Haussmann BIG (2015). No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums. Plant and Soil Published online: 14 March 2015 (DOI: 10.1007/s11104-015-2437-1). Not open access; view abstract. (G7010.03.03)
Leiser WL, Olatoye MO, Rattunde HFW, Neumann G, Weltzien E and Haussmann BIG (2015). No need to breed for enhanced colonization by arbuscular mycorrhizal fungi to improve low-P adaptation of West African sorghums. Plant and Soil Published online: 14 March 2015 (DOI: 10.1007/s11104-015-2437-1). Not open access; view abstract. (G7010.03.03)
Association of mid-reproductive stage canopy temperature depression with the molecular markers and grain yields of chickpea (Cicer arietinum L.) germplasm under terminal drought
Purushothaman R, Thudi M, Krishnamurthy L, Upadhyaya HD, Kashiwagi J, Gowda CLL and Varshney RK (2015). Association of mid-reproductive stage canopy temperature depression with the molecular markers and grain yields of chickpea (Cicer arietinum L.) germplasm under terminal drought. Field Crops Research 174:1–11 (DOI: 10.1016/j.fcr.2015.01.007). (G4008-12)
Abstract: Canopy temperature depression (CTD) has been used to estimate crop yield and drought tolerance. However, when to measure CTD for the best breeding selection efficacy has seldom been addressed. The objectives of this study were to evaluate CTD as a drought response measure, identify suitable crop stage for measurement and associated molecular markers. CTD was measured using an infrared camera on 59, 62, 69, 73, 76 and 82 days after sowing (DAS) and the grain yield, shoot biomass and harvest index (%). CTD recorded at 62 DAS was positively associated with the grain yield by 40% and shoot biomass by 27% and such association diminished gradually to minimum after 76 DAS. Moreover, CTD at 62 DAS also showed similar positive association with the grain yield recorded in two previous years (r = 0.45***, 0.42***). Genome-wide and candidate gene based association analysis had revealed the presence of nine SSR, 11 DArT and three gene-based markers that varied across the six stages of observation. Two SSR markers were associated with CTD through crop phenology or grain yield while the rest were associated only with CTD for computing marker-trait associations (MTAs). The phenotypic variation explained by the markers was the highest at 62 DAS. These results confirm the importance of continued transpiration and the ability of the roots to supply stored soil water under terminal drought. The selection for grain yield through CTD is done best 15 days after the mean flowering time.
Purushothaman R, Thudi M, Krishnamurthy L, Upadhyaya HD, Kashiwagi J, Gowda CLL and Varshney RK (2015). Association of mid-reproductive stage canopy temperature depression with the molecular markers and grain yields of chickpea (Cicer arietinum L.) germplasm under terminal drought. Field Crops Research 174:1–11 (DOI: 10.1016/j.fcr.2015.01.007). (G4008-12)
Abstract: Canopy temperature depression (CTD) has been used to estimate crop yield and drought tolerance. However, when to measure CTD for the best breeding selection efficacy has seldom been addressed. The objectives of this study were to evaluate CTD as a drought response measure, identify suitable crop stage for measurement and associated molecular markers. CTD was measured using an infrared camera on 59, 62, 69, 73, 76 and 82 days after sowing (DAS) and the grain yield, shoot biomass and harvest index (%). CTD recorded at 62 DAS was positively associated with the grain yield by 40% and shoot biomass by 27% and such association diminished gradually to minimum after 76 DAS. Moreover, CTD at 62 DAS also showed similar positive association with the grain yield recorded in two previous years (r = 0.45***, 0.42***). Genome-wide and candidate gene based association analysis had revealed the presence of nine SSR, 11 DArT and three gene-based markers that varied across the six stages of observation. Two SSR markers were associated with CTD through crop phenology or grain yield while the rest were associated only with CTD for computing marker-trait associations (MTAs). The phenotypic variation explained by the markers was the highest at 62 DAS. These results confirm the importance of continued transpiration and the ability of the roots to supply stored soil water under terminal drought. The selection for grain yield through CTD is done best 15 days after the mean flowering time.
Full article
Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids
Leal-Bertioli SCM, Santos SP, Dantas KM, Inglis PW, Nielen S, Araujo ACG, Silva JP, Cavalcante U, Guimarães PM, Brasileiro ACM, Carrasquilla-Garcia N, Penmetsa RV, Cook D, Moretzsohn MC and Bertioli DJ (2015). Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids. Annals of Botany 115(2):237–49 (DOI: 10.1093/aob/mcu237). First published online in December 2014. (G6010.01)
Abstract: Background and Aims Arachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with an AABB genome. Arachis batizocoi was once considered the ancestral donor of the peanut B genome, but cytogenetics and DNA phylogenies have indicated a new genome classification, ‘K’. These observations seem inconsistent with genetic studies and breeding that have shown that A. batizocoi can behave as a B genome.
Methods The genetic behaviour, genome composition and phylogenetic position of A. batizocoi were studied using controlled hybridizations, induced tetraploidy, whole-genome in situ fluorescent hybridization (GISH) and molecular phylogenetics.
Key Results Sterile diploid hybrids containing AK genomes were obtained using A. batizocoi and the A genome species A. duranensis, A. stenosperma, A. correntina or A. villosa. From these, three types of AAKK allotetraploids were obtained, each in multiple independent polyploidy events. Induced allotetraploids were vigorous and fertile, and were hybridized to A. hypogaea to produce F1 hybrids. Even with the same parental combination, fertility of these F1 hybrids varied greatly, suggesting the influence of stochastic genetic or epigenetic events. Interestingly, hybrids with A. hypogaea ssp. hypogaea were significantly more fertile than those with the subspecies fastigiata. GISH in cultivated × induced allotetraploids hybrids (harbouring AABK genomes) and a molecular phylogeny using 16 intron sequences showed that the K genome is distinct, but more closely related to the B than to the A genome.
Conclusions The K genome of A. batizocoi is more related to B than to the A genome, but is distinct. As such, when incorporated in an induced allotetraploid (AAKK) it can behave as a B genome in crosses with peanut. However, the fertility of hybrids and their progeny depends upon the compatibility of the A genome interactions. The genetic distinctness of A. batizocoi makes it an important source of allelic diversity in itself, especially in crosses involving A. hypogaea ssp. hypogaea.
Leal-Bertioli SCM, Santos SP, Dantas KM, Inglis PW, Nielen S, Araujo ACG, Silva JP, Cavalcante U, Guimarães PM, Brasileiro ACM, Carrasquilla-Garcia N, Penmetsa RV, Cook D, Moretzsohn MC and Bertioli DJ (2015). Arachis batizocoi: a study of its relationship to cultivated peanut (A. hypogaea) and its potential for introgression of wild genes into the peanut crop using induced allotetraploids. Annals of Botany 115(2):237–49 (DOI: 10.1093/aob/mcu237). First published online in December 2014. (G6010.01)
Abstract: Background and Aims Arachis batizocoi is a wild relative of cultivated peanut (A. hypogaea), an allotetraploid with an AABB genome. Arachis batizocoi was once considered the ancestral donor of the peanut B genome, but cytogenetics and DNA phylogenies have indicated a new genome classification, ‘K’. These observations seem inconsistent with genetic studies and breeding that have shown that A. batizocoi can behave as a B genome.
Methods The genetic behaviour, genome composition and phylogenetic position of A. batizocoi were studied using controlled hybridizations, induced tetraploidy, whole-genome in situ fluorescent hybridization (GISH) and molecular phylogenetics.
Key Results Sterile diploid hybrids containing AK genomes were obtained using A. batizocoi and the A genome species A. duranensis, A. stenosperma, A. correntina or A. villosa. From these, three types of AAKK allotetraploids were obtained, each in multiple independent polyploidy events. Induced allotetraploids were vigorous and fertile, and were hybridized to A. hypogaea to produce F1 hybrids. Even with the same parental combination, fertility of these F1 hybrids varied greatly, suggesting the influence of stochastic genetic or epigenetic events. Interestingly, hybrids with A. hypogaea ssp. hypogaea were significantly more fertile than those with the subspecies fastigiata. GISH in cultivated × induced allotetraploids hybrids (harbouring AABK genomes) and a molecular phylogeny using 16 intron sequences showed that the K genome is distinct, but more closely related to the B than to the A genome.
Conclusions The K genome of A. batizocoi is more related to B than to the A genome, but is distinct. As such, when incorporated in an induced allotetraploid (AAKK) it can behave as a B genome in crosses with peanut. However, the fertility of hybrids and their progeny depends upon the compatibility of the A genome interactions. The genetic distinctness of A. batizocoi makes it an important source of allelic diversity in itself, especially in crosses involving A. hypogaea ssp. hypogaea.
Phosphorous efficiency and tolerance traits for selection of sorghum for performance in phosphorous-limited environments
Leiser WL, Rattunde HFW, Piepho H-P, Weltzien E, Diallo A, Toure A and Hausmann BIG (2015). Phosphorous efficiency and tolerance traits for selection of sorghum for performance in phosphorous-limited environments. Crop Science 55 Published online: 27 March 2015 (DOI: 10.2135/cropsci2014.05.0392). (G7010.03.03)
Abstract: Sorghum (Sorghum bicolor (L.) Moench) is widely cultivated in West Africa (WA) on soils with low phosphorus (P) availability. Large genetic variation for grain yield (GY) under low-P conditions was observed among WA sorghum genotypes, but information is lacking on the usefulness of P-tolerance ratios (relative performance in –P [no P fertilizer] vs. +P [with P fertilizer] conditions) and measures of P-acquisition and internal P-use efficiency as selection criteria for enhancing GY under low-P conditions. We evaluated 70 WA sorghum genotypes for GY performance under −P and +P conditions for 5 yr in two locations in Mali and assessed P acquisition (e.g., P content in biomass) and P-use efficiency (e.g., grain produced per unit P uptake) traits under −P and +P conditions in one site in 2010. Significant genetic variation existed for all P-tolerance ratios across multiple sites. Photoperiod-sensitive landrace genotypes showed significantly better P tolerance and less delay of heading under P-limited conditions compared with photoperiod-insensitive varieties. Genotypic correlations of P-tolerance ratios to GY under −P were moderate. Phosphorous acquisition and P-use efficiency traits independent of harvest index were of similar importance for GY under −P conditions in statistically independent trials. However grain-P and stover-P concentrations from one −P trial showed only weak correlations with GYs in statistically independent trials. Highest predicted gains for −P GY were obtained by theoretical index selection based on −P GY combined with P-use efficiency traits (e.g., low-grain P concentration). Such index selection is expected to achieve both increased sorghum productivity and P sustainability in the P-limited WA production systems.
Leiser WL, Rattunde HFW, Piepho H-P, Weltzien E, Diallo A, Toure A and Hausmann BIG (2015). Phosphorous efficiency and tolerance traits for selection of sorghum for performance in phosphorous-limited environments. Crop Science 55 Published online: 27 March 2015 (DOI: 10.2135/cropsci2014.05.0392). (G7010.03.03)
Abstract: Sorghum (Sorghum bicolor (L.) Moench) is widely cultivated in West Africa (WA) on soils with low phosphorus (P) availability. Large genetic variation for grain yield (GY) under low-P conditions was observed among WA sorghum genotypes, but information is lacking on the usefulness of P-tolerance ratios (relative performance in –P [no P fertilizer] vs. +P [with P fertilizer] conditions) and measures of P-acquisition and internal P-use efficiency as selection criteria for enhancing GY under low-P conditions. We evaluated 70 WA sorghum genotypes for GY performance under −P and +P conditions for 5 yr in two locations in Mali and assessed P acquisition (e.g., P content in biomass) and P-use efficiency (e.g., grain produced per unit P uptake) traits under −P and +P conditions in one site in 2010. Significant genetic variation existed for all P-tolerance ratios across multiple sites. Photoperiod-sensitive landrace genotypes showed significantly better P tolerance and less delay of heading under P-limited conditions compared with photoperiod-insensitive varieties. Genotypic correlations of P-tolerance ratios to GY under −P were moderate. Phosphorous acquisition and P-use efficiency traits independent of harvest index were of similar importance for GY under −P conditions in statistically independent trials. However grain-P and stover-P concentrations from one −P trial showed only weak correlations with GYs in statistically independent trials. Highest predicted gains for −P GY were obtained by theoretical index selection based on −P GY combined with P-use efficiency traits (e.g., low-grain P concentration). Such index selection is expected to achieve both increased sorghum productivity and P sustainability in the P-limited WA production systems.
Registration of the Ki14 × B73 recombinant inbred mapping population of maize
Pratt RC, Holland JB, Balint-Kurti PJ, Coles ND, Zwonitzer JC, Casey MA and McMullens MC (2015). Registration of the Ki14 × B73 recombinant inbred mapping population of maize. Journal of Plant Registrations 9(2):262–265 (DOI: 10.3198/jpr2014.06.0041crmp). Not open access; view abstract.
Pratt RC, Holland JB, Balint-Kurti PJ, Coles ND, Zwonitzer JC, Casey MA and McMullens MC (2015). Registration of the Ki14 × B73 recombinant inbred mapping population of maize. Journal of Plant Registrations 9(2):262–265 (DOI: 10.3198/jpr2014.06.0041crmp). Not open access; view abstract.
Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis
Leal-Bertioli S, Shirasawa K, Abernathy B, Moretzsohn M, Chavarro C, Clevenger J, Ozias-Akins P, Jackson S and Bertioli D (2015). Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis. Genetics 199(4):1093–1105 (DOI: 10.1534/genetics.115.174607). (G6010.01)
Abstract: Arachis hypogaea L. (cultivated peanut) is an allotetraploid (2n = 4x = 40) with an AABB genome type. Based on cytogenetic studies it has been assumed that peanut and wild-derived induced AABB allotetraploids have classic allotetraploid genetic behavior with diploid-like disomic recombination only between homologous chromosomes, at the exclusion of recombination between homeologous chromosomes. Using this assumption, numerous linkage map and quantitative trait loci studies have been carried out. Here, with a systematic analysis of genotyping and gene expression data, we show that this assumption is not entirely valid. In fact, autotetraploid-like tetrasomic recombination is surprisingly frequent in recombinant inbred lines generated from a cross of cultivated peanut and an induced allotetraploid derived from peanut's most probable ancestral species. We suggest that a better, more predictive genetic model for peanut is that of a "segmental allotetraploid" with partly disomic, partly tetrasomic genetic behavior. This intermediate genetic behavior has probably had a previously overseen, but significant, impact on the genome and genetics of cultivated peanut.
Leal-Bertioli S, Shirasawa K, Abernathy B, Moretzsohn M, Chavarro C, Clevenger J, Ozias-Akins P, Jackson S and Bertioli D (2015). Tetrasomic recombination is surprisingly frequent in allotetraploid Arachis. Genetics 199(4):1093–1105 (DOI: 10.1534/genetics.115.174607). (G6010.01)
Abstract: Arachis hypogaea L. (cultivated peanut) is an allotetraploid (2n = 4x = 40) with an AABB genome type. Based on cytogenetic studies it has been assumed that peanut and wild-derived induced AABB allotetraploids have classic allotetraploid genetic behavior with diploid-like disomic recombination only between homologous chromosomes, at the exclusion of recombination between homeologous chromosomes. Using this assumption, numerous linkage map and quantitative trait loci studies have been carried out. Here, with a systematic analysis of genotyping and gene expression data, we show that this assumption is not entirely valid. In fact, autotetraploid-like tetrasomic recombination is surprisingly frequent in recombinant inbred lines generated from a cross of cultivated peanut and an induced allotetraploid derived from peanut's most probable ancestral species. We suggest that a better, more predictive genetic model for peanut is that of a "segmental allotetraploid" with partly disomic, partly tetrasomic genetic behavior. This intermediate genetic behavior has probably had a previously overseen, but significant, impact on the genome and genetics of cultivated peanut.
Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress
Wang SG, Jia SS, Sun DZ, Wang HY, Dong FF, Ma HX, Jing RL and Ma G (2015). Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress. Photosynthetica 53(2):299–305 (DOI: 10.1007/s11099-015-0114-5). Not open access; view abstract. (G7010.02.01)
Wang SG, Jia SS, Sun DZ, Wang HY, Dong FF, Ma HX, Jing RL and Ma G (2015). Genetic basis of traits related to stomatal conductance in wheat cultivars in response to drought stress. Photosynthetica 53(2):299–305 (DOI: 10.1007/s11099-015-0114-5). Not open access; view abstract. (G7010.02.01)
Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes
Brasileiro ACM, Morgante CV, Araujo ACG, Leal-Bertioli SCM, Silva AK, Martins ACQ, Vinson CC, Santos CMR, Bonfim O, Togawa RC, Saraiva MAP, Bertioli DJ and Guimaraes PM (2015). Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant Molecular Biology Reporter Published online: 11 April 2015 (DOI 10.1007/s11105-015-0882-x). (G6010.01)
Abstract: Peanut (Arachis hypogaea L.) is an important legume cultivated mostly in drought-prone areas where its productivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for peanut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysis from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC, and bZIP transcription factors, displaying significant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recovery in wild Arachis species. The generated transcriptome data, besides being a valuable resource for gene discovery, will allow the characterization of new alleles and development of molecular markers associated with drought responses in peanut. These together constitute important tools for the peanut breeding program and also contribute to a better comprehension of gene modulation in response to water deficit and rehydration.
Brasileiro ACM, Morgante CV, Araujo ACG, Leal-Bertioli SCM, Silva AK, Martins ACQ, Vinson CC, Santos CMR, Bonfim O, Togawa RC, Saraiva MAP, Bertioli DJ and Guimaraes PM (2015). Transcriptome profiling of wild Arachis from water-limited environments uncovers drought tolerance candidate genes. Plant Molecular Biology Reporter Published online: 11 April 2015 (DOI 10.1007/s11105-015-0882-x). (G6010.01)
Abstract: Peanut (Arachis hypogaea L.) is an important legume cultivated mostly in drought-prone areas where its productivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for peanut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysis from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC, and bZIP transcription factors, displaying significant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recovery in wild Arachis species. The generated transcriptome data, besides being a valuable resource for gene discovery, will allow the characterization of new alleles and development of molecular markers associated with drought responses in peanut. These together constitute important tools for the peanut breeding program and also contribute to a better comprehension of gene modulation in response to water deficit and rehydration.
Physiological mechanisms contributing to the QTL-combination effects on improved performance of IR64 rice NILs under drought
Henry A, Swamy BPM, Dixit S, Torres RD, Batoto TC, Manalili M, Anantha MS, Mandal NP and Kumar A (2015). Physiological mechanisms contributing to the QTL-combination effects on improved performance of IR64 rice NILs under drought. Journal of Experimental Botany 66(7):1787–1799 (DOI: 10.1093/jxb/eru506). (G3008.06)
Abstract: Characterizing the physiological mechanisms behind major-effect drought-yield quantitative trait loci (QTLs) can provide an understanding of the function of the QTLs—as well as plant responses to drought in general. In this study, we characterized rice (Oryza sativa L.) genotypes with QTLs derived from drought-tolerant traditional variety AdaySel that were introgressed into drought-susceptible high-yielding variety IR64, one of the most popular megavarieties in South Asian rainfed lowland systems. Of the different combinations of the four QTLs evaluated, genotypes with two QTLs (qDTY2.2 + qDTY4.1) showed the greatest degree of improvement under drought compared with IR64 in terms of yield, canopy temperature, and normalized difference vegetation index (NDVI). Furthermore, qDTY2.2 and qDTY4.1 showed a potential for complementarity in that they were each most effective under different severities of drought stress. Multiple drought-response mechanisms were observed to be conferred in the genotypes with the two-QTL combination: higher root hydraulic conductivity and in some cases greater root growth at depth. As evidenced by multiple leaf water status and plant growth indicators, these traits affected transpiration but not transpiration efficiency or harvest index. The results from this study highlight the complex interactions among major-effect drought-yield QTLs and the drought-response traits they confer, and the need to evaluate the optimal combinations of QTLs that complement each other when present in a common genetic background.
Henry A, Swamy BPM, Dixit S, Torres RD, Batoto TC, Manalili M, Anantha MS, Mandal NP and Kumar A (2015). Physiological mechanisms contributing to the QTL-combination effects on improved performance of IR64 rice NILs under drought. Journal of Experimental Botany 66(7):1787–1799 (DOI: 10.1093/jxb/eru506). (G3008.06)
Abstract: Characterizing the physiological mechanisms behind major-effect drought-yield quantitative trait loci (QTLs) can provide an understanding of the function of the QTLs—as well as plant responses to drought in general. In this study, we characterized rice (Oryza sativa L.) genotypes with QTLs derived from drought-tolerant traditional variety AdaySel that were introgressed into drought-susceptible high-yielding variety IR64, one of the most popular megavarieties in South Asian rainfed lowland systems. Of the different combinations of the four QTLs evaluated, genotypes with two QTLs (qDTY2.2 + qDTY4.1) showed the greatest degree of improvement under drought compared with IR64 in terms of yield, canopy temperature, and normalized difference vegetation index (NDVI). Furthermore, qDTY2.2 and qDTY4.1 showed a potential for complementarity in that they were each most effective under different severities of drought stress. Multiple drought-response mechanisms were observed to be conferred in the genotypes with the two-QTL combination: higher root hydraulic conductivity and in some cases greater root growth at depth. As evidenced by multiple leaf water status and plant growth indicators, these traits affected transpiration but not transpiration efficiency or harvest index. The results from this study highlight the complex interactions among major-effect drought-yield QTLs and the drought-response traits they confer, and the need to evaluate the optimal combinations of QTLs that complement each other when present in a common genetic background.
Genotypic variation in grain yield and flowering pattern in terminal and intermittent drought screening methods in rainfed lowland rice
Monkham T, Jongdee B, Pantuwan G, Sanitchon J, Mitchell JH and Fukai S (2015). Genotypic variation in grain yield and flowering pattern in terminal and intermittent drought screening methods in rainfed lowland rice. Field Crops Research 175:26–36 (DOI: 10.1016/j.fcr.2015.02.003). Not open access; view abstract. (G3008.06)
Monkham T, Jongdee B, Pantuwan G, Sanitchon J, Mitchell JH and Fukai S (2015). Genotypic variation in grain yield and flowering pattern in terminal and intermittent drought screening methods in rainfed lowland rice. Field Crops Research 175:26–36 (DOI: 10.1016/j.fcr.2015.02.003). Not open access; view abstract. (G3008.06)
