It was his commitment to helping farmers that led Chiedozie to forsake his dream of becoming a surgeon, and instead to train as a plant breeder and help smallholder farmers in Nigeria. Having grown up in a small town in south-eastern Nigeria where poverty was a daily reality, he was particularly concerned about food security and nutrition for the people. He dreamt of developing cassava varieties that could beat the pests and diseases that often devastate such crops.
Peeling cassava roots.
“The food people grow should be nutritious, resistant and high-yielding enough to allow them to sell some of it and make money for other things in life, such as building a house, getting a motorbike or sending their kids to school,” Chiedozie says.
Nigeria is the most populous African country, with a population of more than 174 million. The main staple food is cassava, making Nigeria the world’s largest producer and consumer of the crop. But cassava is also important in other African countries. It is grown by nearly every farming family in sub-Saharan Africa. Africa produced nearly 140 million metric tonnes of cassava in 2012 – but most of the production is low-yielding subsistence farming for food by small-scale farmers for food for their own households alone.
After almost eight years working on GCP-supported cassava projects, Chiedozie is proud of what they have managed to accomplish: “That we’ve been able to give African farmers the varieties that they will love to grow is my biggest achievement”.
Meet Chiedozie and hear all about his research and the importance of cassava in the video series below (or watch on YouTube):
Transformation for Chiedozie – and for cassava
Chiedozie’s journey with GCP began after he had gained his PhD in yam breeding from the University of Ibadan, Nigeria. He undertook further studies and training at Cornell University and the University of Washington, both in the USA. He then returned home to Nigeria to lead the cassava breeding team at the National Root Crops Research Institute (NRCRI) and, following a promotion in 2010, was made Assistant Director of NRCRI’s Biotechnology Department. In 2004, a chance find on the Internet of a molecular breeding training programme in South Africa first led to Chiedozie’s involvement in GCP.
In 2010, work by Chiedozie and the NRCRI team, in collaboration with a transnational network of partners, resulted in the official release to Nigerian farmers of Africa’s first cassava variety developed using molecular-breeding techniques. Known as UMUCASS33 (or CR 41-10), it was resistant to cassava mosaic disease (CMD) – a devastating plant disease that can wipe out entire cassava crops – and also highly nutritious. In addition to a stream of further disease-resistant varieties, in 2012 they followed this accomplishment with the release of a high-starch variety bred using molecular techniques.
Nigerian women at work processing cassava.
In 2011, the cassava team together with the International Institute of Tropical Agriculture (IITA) and HarvestPlus (another CGIAR Challenge Programme focussed on the nutritional enrichment of crops), released three cassava varieties rich in pro-vitamin A, which hold the potential to provide children under five and women of reproductive age with up to 25 percent of their daily vitamin A requirement – a figure Chiedozie and his team are now ambitiously striving to increase to 50 percent. In 2014, they released three more pro-vitamin A varieties with higher concentrations of beta-carotene.
These new and improved varieties – all generated as a direct or indirect result of his engagement in GCP projects – are, Chiedozie says, worth their weight in gold for the people of Africa.
Research that delivers benefits to poor farmers is what drives Chiedozie. In addition to the direct rewards of new varieties there are other highlights from his involvement with GCP, indicating a long term change in breeding science: “People are now using improved or modern techniques in breeding; people think about database management in cassava breeding across Africa; and African breeders are getting PhDs in molecular breeding.”
Cassava leaves.
Building African capacity
Chiedozie believes a crucial element of GCP’s success in breeding better cassava varieties for smallholder African farmers lies in the capacity building and infrastructure support provided by GCP.
After his initial GCP training at the University of Pretoria, South Africa, Chiedozie engaged in other capacity-building opportunities, including a one-year visiting scientist fellowship at the International Center for Tropical Agriculture (CIAT) in Colombia. The significance of these early GCP opportunities was, Chiedozie says, momentous: “Prior to my GCP work, I was more or less a plant breeder, and a conventional one at that. Whilst I’d been exposed to molecular tools during my early work on yam and other crops, I was not applying them in my work back then.”
Chiedozie emphasises that such training opportunities are vital for the future food security of Africa. “We raised up a new crop of cassava breeders in Africa – people who were bold enough to take up a molecular breeding project and pursue it with support from the international centres. And today we are seeing the results of that. Cassava breeding programmes are standing today because of our quality of seeds sown in the past.”
The networking opportunities offered by the Cassava Community of Practice – founded by GCP and now hosted by the Integrated Breeding Platform (IBP) – have meant that Chiedozie and his colleagues could expand their collaboration at the local, national and regional levels: “We now have a network of cassava breeders that you can count on and relate with in different countries. This has really widened our horizons and also made our work more visible,” he says, citing effective links formed with Côte d’Ivoire, Ethiopia, Ghana, Liberia, Malawi, Mozambique, Sierra Leone and South Sudan.
Selling fufu, a staple food made with cassava flour, at a market in Nigeria.
A paradigm shift
These opportunities have led to what Chiedozie calls a ‘paradigm shift’ in how national research agencies are viewed by donors and research investors: “GCP helped us to build an image for ourselves in Nigeria and in Africa, and this created a confidence in other global actors, who, on seeing our ability to deliver results, are choosing to invest in us.
“Our work with GCP helped us to gain that capacity that we needed to be able to negotiate or even make a request for funding. And people are able to trust that you can deliver if you have delivered in the past for an organisation like GCP. So it gave us credibility; it gives us a platform to be able to speak to donors directly, and donors can now approach us, which never used to happen in pre GCP days.”
Hear from Chiedozie on the beneficial outcomes of GCP – in terms not only of variety releases but also of attracting further projects, prestige, and enthusiastic young breeders – in the video below (or on YouTube):
For Chiedozie, his dream of helping his country’s struggling farmers and people is coming true. He has no regrets about dropping his dream of becoming a surgeon for one of helping his country as a plant breeder: “Coming from Umuahia, a small town in the southeast of the country, I grew up in an environment where you see people who are struggling, weak from disease, poor, and with no opportunities to send their children to school.
“Despite the social injustice around me, I always thought there was opportunity to improve people’s lives. This is what the GCP-supported research has helped me to do, even faster than I would have believed possible.”
View Chiedozie’s 2013 presentation from the GCP General Research Meeting: ‘Improving and deploying markers for biotic stresses in cassava’ (on SlideShare):
Young Nigerian scientists often leave Africa and look for jobs with international research agencies overseas. But with the CGIAR Generation Challenge Programme (GCP)-funded Cassava Research Initiative (RI), two young nationals have been leading the international collaboration and injecting confidence into Africa’s research capacity.
Leadership is a quality admired and consistently sought after, particularly when overcoming a challenge. Some leaders direct from afar; others rise through the ranks and work with their peers on the ground – winning respect from the people they lead as they get their hands dirty.
Dream team: Emmanuel Okogbenin (left) and Chiedozie Egesi (right), both of Nigeria’s National Root Crops Research Institute.
“If you want to work for the people, you have to walk with the people – that’s an African concept,” says Emmanuel Okogbenin, a plant breeder and geneticist at Nigeria’s National Root Crops Research Institute (NRCRI). “Then when you work with the people, you really understand what they want. When you speak, they know they can trust you.”
This powerful sentiment is one reason why GCP sought the collaboration of NRCRI in overcoming the challenge of sustaining Africa’s, and indeed the world’s, cassava production.
Having started as a small farm in 1923, NRCRI has taken giant strides to become one of Nigeria’s best research institutes, contributing immensely to the country’s economic development and making it the leading producer of cassava in the world. NRCRI Executive Director Julius Chukwuma Okonkwo says, “This would not have been attainable if not for the trust and support that GCP had in us when they made two of our young cassava researchers the leaders of an international collaboration.”
The two researchers to whom Julius refers are Emmanuel and his colleague Chiedozie Egesi, also a plant breeder and geneticist at NRCRI. Their combined 36 years’ of cassava research experience is matched by their passion to get the best out of Nigeria’s main staple crop.
And they are happy to get some dirt under their fingernails. “It’s just as important to work with the farmers in the field and understand what they want, as it is to do the research in the lab,” says Emmanuel. “At the end of the day we need to please the farmers, as they are the ones who will be using the new varieties that we are developing to sustain their livelihoods.”
Meet Chiedozie and Emmanuel in the video playlist below, learn more about cassava in Africa, and hear all about their research (or watch on Youtube):
Emmanuel explains that before GCP, “most African national programmes didn’t really have established crop-breeding programmes, and didn’t have the resources” to do the scale of research GCP assisted with. Nor did they have the capacity to use molecular-breeding techniques, which can potentially halve the time it takes to develop new varieties.
With help from GCP and CIAT, NRCRI was able to equip a new molecular-breeding laboratory, and staff were trained to incorporate molecular-breeding techniques into their breeding programme. “GCP was there not only to provide technology, but also to guide us in how to operate that technology,” explains Chiedozie.
Julius points out that both Chiedozie and Emmanuel were also influential in disseminating this knowledge and, in turn, building and sustaining NRCRI’s human capacity. “They both mentored many young scientists who have chosen a career in cassava and molecular breeding because of this.”
Transporting a bountiful cassava harvest from farm to market in Nigeria.
With training and infrastructure in place, NRCRI led an international collaboration that in 2010 released Africa’s first cassava variety developed using molecular-breeding techniques. Known as UMUCASS33 (or CR 41-10), it was resistant to cassava mosaic disease (CMD) – a devastating plant disease that can wipe out farmers’ entire cassava crops – and also highly nutritious. This was swiftly followed by a second similar variety, CR 36-5, and supplied to farmers.
Between this landmark release and GCP’s close in 2014, the cassava team had already released nearly 20 higher yielding, more nutritious varieties resistant to diseases and pests, and had begun working on developing drought-tolerant varieties.
These new and improved varieties – all generated as a direct or indirect result of his engagement in GCP projects – are, Chiedozie says, worth their weight in gold: “Through these materials, people’s livelihoods can be improved. The food people grow should be nutritious, resistant and high-yielding enough to allow them to sell some of it and make money for other things in life, such as building a house, getting a motorbike or sending their kids to school.” This social aspect is particularly pertinent in Nigeria, where these cassava varieties will have the greatest impact.
Feeding a giant
Nigerian farmer with his bountiful cassava harvest.
Nigeria is often referred to as the ‘Giant of Africa’. It is the most populous African country, with over 174 million inhabitants. The population’s main staple food is cassava, making Nigeria the world’s largest producer and consumer of the crop. At the same time, the country imports almost USD 4 billion of wheat every year – a figure that is expected to quadruple by 2030 if wheat consumption continues to grow at the same rate it is today.
The government is wary of this ‘overreliance’ on imported grain and is working towards making the country less reliant on wheat by imposing a wheat tariff. It also hopes to boost cassava production and commercialisation by promoting 20 percent substitution of cassava flour for wheat in breadmaking.
“The government feels that to quickly change the fortunes of farmers, cassava is the way to go,” explains Emmanuel, who liaises with the Nigerian Government to promote to farmers the benefit of cassava varieties with high starch concentrations. It is the flour from these varieties that is being used to partially replace wheat flour to make bread. GCP support has been crucial here too, in providing vital scientific information to the government. Emmanuel explains: “The tariff from wheat is expected to be ploughed back to support agricultural development – especially in the cassava sector – as the government seeks to increase cassava production to support flour mills.”
Cassava offers a huge opportunity to transform the agricultural economy, stimulate rural development and further improve Nigeria’s gross domestic product. In 2014, Nigeria’s economy surpassed that of South Africa’s to become the largest on the continent. By 2050, Nigeria is expected to rise further and become one of the world’s top 20 economies.
Unfortunately, however, like many growing economies worldwide, Nigeria is still working to address severe inequality, including in the distribution of wealth and in feeding the country’s expanding population.
A woman with her children at work in a cassava processing centre in Nigeria.
It’s a problem Chiedozie understands well: “Nigeria is an oil-producing country, but you still see grinding poverty in some cases,” he says. “Coming from a small town in the southeast of the country, I grew up in an environment where you see people who are struggling, weak from disease, poor, and with no opportunities to send their children to school,” he reveals. The poverty challenge, he explains, hits smallholder farmers particularly hard: “Urban development caught up with them in the end: some of them don’t even have access to the land that they inherited, so they’re forced to farm along the street.”
For Chiedozie, the seemingly bleak picture only served to ignite a fierce determination and motivation to act: “Despite the social injustice around me, I always thought there was opportunity to improve people’s lives.” And thus galvanised by the plight of Nigerian farmers, Chiedozie promptly shelved his plans for a career in medical surgery and pursued biological sciences and a PhD in crop genetics, a course he interspersed with training stints in the USA at Cornell University and the University of Washington, before returning to his homeland to accept a job as head of the cassava breeding team, and – following a promotion in 2010 – to become Assistant Director of the Biotechnology Department at NRCRI.
Empowering African researchers
Carrying cassava at a processing centre in Nigeria.
Emmanuel, who followed a similar educational route to Chiedozie, says both he and his colleague are exceptions to the norm in Africa, where African researchers tend to look for opportunities at international or private institutes rather than in national breeding programmes.
“It is difficult being a researcher in Africa,” says Emmanuel. “We don’t get paid as much as breeders in more developed countries, and funding is very hard to obtain.”
Emmanuel says his proudest moment was when GCP was looking for Africans to take up leadership roles. “They felt we could change things around and set a precedent to bring people back to the continent,” he says. “They appreciated our values and the need to install African leaders on the ground in Africa rather than in Europe, Asia or the Americas.”
Jean-Marcel Ribaut, GCP’s Director, says that seeking this local leadership was a novel approach for a transnational programme like GCP at the time, and proved to be an imperative feature for all GCP Research Initiatives. “The reasoning behind the approach is two-fold: Firstly, it’s important that our national partners share in feeling ownership of the projects and outcomes; secondly, they are gaining experience in the role so they can continue to do so after the close of the Programme in 2014,” he says. “We feel that most of our leading institutes, NRCRI included, are in a better position now than when they joined the project, and that this, along with their experience, has already gained them more exposure and funding opportunities.”
This is indeed true of the NRCRI cassava team, which is engaging with the Bill & Melinda Gates Foundation, Cornell University, IITA and Uganda’s National Crop Resources Research Institute in an initiative that Chiedozie promises will be at the front of cutting-edge technology. “We are still working out specifics, but it will see us continuing to use marker-assisted breeding techniques to develop higher yielding, stress-tolerant cassava varieties.”
Chiedozie adds this would not have been possible without GCP, which helped them to develop their capacity in Nigeria and in Africa, and this has “created a confidence in other global actors, who, on seeing our ability to deliver results, are choosing to invest in us.”
Before GCP came along, cassava was something of an orphan crop in agricultural research. Among the challenges to efficient breeding of cassava are that it is slow to grow and is propagated, not by seed, but using cut sections of stem like those shown. But with investment and capacity building from GCP, particularly in molecular breeding tools, African cassava scientists have gained a new confidence and prestige.
Continuing the momentum
One organisation that has been impressed by the work done at NRCRI is the CGIAR Research Program on Roots, Tubers and Bananas (RTB). RTB Director Graham Thiele has been following the work done at NRCRI since 2010 with great interest. “We have been really impressed to see a national programme like NRCRI playing a leading role in these successful GCP projects, and grow as a result of this,” he says.
One area of research that has particularly impressed Graham is Chiedozie and Emmanuel’s pre-emptive breeding for cassava brown streak disease (CBSD) resistance. “CBSD isn’t currently an issue in Nigeria but it has the potential to wipe out all crops, as it has in Uganda and Tanzania, if it continues to spread west from these countries,” he explains.
“What Chiedozie and Emmanuel are doing is using molecular markers, developed in collaboration with IITA, to search for genes in their varieties that confer resistance to brown streak virus. They can then use these when breeding for CBSD resistance without exposing cassava to the virus. It’s very exciting and forward thinking, as normally people breed for resistance only when the disasters happen.”
As GCP approached its sunset in December 2014, Chiedozie and Emmanuel were reaching out to RTB to seek funding to continue this and other projects they are currently working on. “They’ve already created some great varieties but have plenty more in the pipeline, so we want to help them finish this work and, most importantly, keep the momentum going,” says Graham.
Chiedozie looks forward to the next steps with optimism, confirming that the new collaboration will continue in the quest to “give African farmers varieties of cassava that they will love to grow.”
Beyond the glittering coastline of what was once known as the Gold Coast, Ghana’s shrublands and rich forested hills are split by forking rivers that reach inland through the country’s lush tropics, into drier western Africa. In the past 40 years, seven major droughts have battered the people of Africa – with the most significant and devastating occurring in the Sahel region and the Horn of Africa in the early 1970s and 1980s.
This little girl in Kenya already seems to know that cassava roots are precious.
But despite the massive social disruption and human suffering that these droughts cause, life goes on. In south-eastern Ghana and in Togo, the three-million-plus people who speak the Ewe language have a word for this. It is agbeli: ‘There is life’. It is no coincidence that this word is also their name for a tropical and subtropical crop that survives through the worst times to feed Africa’s families: cassava.
Cassava is a lifeline for African people, and is a particularly important staple food for poorer farmers. More cassava is produced in Africa than any other crop, and it is grown by nearly every farming family in sub-Saharan Africa, supplying about a third of the region’s daily energy intake. In the centuries since Portuguese traders introduced this Amazonian plant to Africa, cassava has flourished, yielding up to 40 tonnes per hectare.
Hear more on just why cassava is so important to food security from Emmanuel Okogbenin, of Nigeria’s National Root Crops Research Institute, in the video below (or watch on Youtube):
African countries produced nearly 140 million tonnes of cassava in 2012 – but most of the production is subsistence farming by small-scale farmers. Even the undisputed global cassava giant, Nigeria, currently produces only just enough to feed its population – and although the country is increasingly moving towards production of cassava for export as an industrial raw material, the poorest farmers often do not produce enough to sell, or have access to these markets.
Because cassava does so well on poor soils, on marginal land and with little rainfall, it can outlast its more sophisticated crop competitors: wheat, rice and maize. In fact, under harsh conditions such as drought, the amount of energy a given area of cassava plants can produce in the form of starchy carbohydrates outstrips all other crops. Chiedozie Egesi, a plant breeder and geneticist at Nigeria’s National Root Crops Research Institute (NRCRI), describes cassava as “the crop you can bet on when every other thing is failing”.
Despite cassava’s superhero cape, however, there’s no denying that its production is not at its highest when faced with diseases, pests, low-nutrient soils and drought. How plants deal with problems like low nutrients or dry conditions is called ‘stress tolerance’ by scientists. Improving this tolerance – plus resistance to diseases and pests – is the long-term goal for staple crops around the world so that they have higher yields in the face of capricious weather and evolving threats.
In the 1980s, catastrophe struck cassava production in East and Central Africa. A serious outbreak of cassava mosaic disease (CMD) – which first slowly shrivels and yellows cassava leaves, then its roots – lasted for almost 15 years and nearly halved cassava yields in that time. Food shortages led to localised famines in 1993 and 1997.
Other diseases affecting cassava include cassava brown streak disease (CBSD), cassava bacterial blight, cassava anthracnose disease and root rot. CBSD is impossible to detect above ground. Its damage is revealed only after harvest, when it can be seen that the creeping brown lesions have spoilt the white flesh of the tubers, rendering them inedible. Many cassava diseases are transmitted through infected cuttings, affecting the next generation in the next season. Pests that also prey on cassava include the cassava green mite and the variegated grasshopper.
Between the effects of drought, diseases, pests and low soil nutrients, cassava yields vary widely – losses can total between 50 and 100 percent in the worst times.
Symptoms of cassava mosaic disease (CMD) and cassava brown streak disease (CBSD), both of which can cripple cassava yields.
GCP takes the first steps to kick start cassava research
The next step forward for cassava appeared to be research towards breeding stronger and more resilient cassava varieties. However, cassava research had long been neglected – scientists say it’s a tricky crop that has garnered far less policy, scientific and monetary interest than the comparatively glamorous crops of maize, rice and wheat.
Watch as Emmanuel tells us more about the complexities and challenges of cassava breeding in the video below (or on YouTube):
Cassava is a plant which ‘drags its feet’: creating new plants has to be done from cuttings, which are costly to cut and handle and don’t store well; the plant takes up to two years to grow to maturity; and it is onerous to harvest. Elizabeth Parkes, of Ghana’s Crops Research Institute (CRI) (currently on secondment at the International Institute of Tropical Agriculture, IITA), says the long wait can be difficult.
This is where the work of scientists funded by the CGIAR Generation Challenge Programme (GCP) came in. Plant breeder and molecular geneticist Emmanuel Okogbenin of NRCRI led the cassava research push launched in 2010. He explains that before GCP, “most national programmes didn’t really have established crop breeding programmes, and didn’t have the manpower” to do the scale of research GCP supported.
Usually, researchers looking to breed crops that are more resistant to drought, diseases and pests would use conventional breeding methods that could take considerable time to deliver any results, especially given cassava’s slow path to maturity. Researchers would be trying to select disease- and pest-resistant plants by looking at how they’re growing in the field, without any way to see the different genetic strengths each plant has.
An IITA researcher exams cassava roots in the field.
This is where new ‘molecular breeding’ tools are particularly useful, given that – genetically – cassava presents more of a challenge to breeders than its cereal counterparts. Like many other vegetatively propagated crops, cassava is highly heterozygous, meaning that the counterpart genes on paired chromosomes tend to be different versions, or alleles, rather than the same. This makes it difficult to identify good parent plants for breeding and, after these are crossed, to accurately select progeny with desired traits. Useful – or detrimental – genes can be present in a cassava plant’s genetic code but not reflected in the plant itself, making breeding more unpredictable – and adding extra obstacles to the hunt for the exact genes that code for better varieties of cassava.
Although late to the world of molecular breeding, cassava had not missed its chance. Guided by GCP’s ambitious remit to increase food security through modern crop breeding, GCP-supported scientists have applied and developed molecular breeding methods that shorten the breeding process by identifying which plants have good genes, even before starting on that long cassava growth cycle. Increasing the capacity of local plant breeders to apply these methods has great potential for delivering better varieties to farmers much faster than has traditionally been the case.
Charting cassava’s genetic material was the first step in the researchers’ molecular quest. Part of the challenge for African and South American researchers was to create a genetic map of the cassava genome. Emmanuel describes the strong foundation that these early researchers built for those coming after: “It was significant when the first draft of the cassava genome sequence was released. It enabled rapid progress in cassava research activities and outcomes, both for GCP and cassava researchers worldwide.”
Cassava on sale in Kampala, Uganda.
Once cassava’s genome had been mapped, the pace picked up. In just one year, GCP-supported scientists phenotyped and genotyped more than 1000 genetically different cassava plants – that is, measured and collected a large amount of information about both their physical and their genetic traits – searching for ‘superstar’ plants with resistance to more than one crop threat. During this process, scientists compare plant’s physical characteristics with their genetic makeup, looking for correlations that reveal regions of the DNA that seem to contain genes that confer traits they are looking for, such as resistance to a particular disease. Within these, scientists then identify sequences of DNA, or ‘molecular markers’, associated with these valuable genes or genetic regions.
Plant breeders can use this knowledge to apply an approach known as marker-assisted selection, choosing their breeding crosses based directly on which plants contain useful genes, using markers like tags. This makes producing better plant varieties dramatically faster and more efficient. “It narrowed the search at an early stage,” explains Emmanuel, “so we could select only material that displayed markers for the genetic traits we’re looking for. There is no longer any need to ship in tonnes of plant material to Africa.”
Like breadcrumbs leading to a clue, breeders use markers to lead to identifying actual genes (rather than just a site on the genome) that give certain plants desirable characteristics. However, this is a particularly difficult process in cassava. Genes are often obscured, partly due to cassava’s highly heterozygous nature. In trials in Africa, where CMD is widespread, resistant types were hard to spot when challenged with the disease, and reliably resistant parents were hard to pin down.
This meant that two decades of screening cassava varieties from South America – where CMD does not yet exist yet – had identified no CMD-resistance genes. But screening of cassava from Nigeria eventually yielded markers for a CMD-resistance gene – a great success for the international collaborative team led by Martin Fregene, who was based in Colombia at the International Center for Tropical Agriculture (CIAT).
This finding was a win for African plant breeders, as it meant they could use molecular breeding to combine the genes producing high-quality and high-yielding cassava from South America with the CMD-resistance gene found in cassava growing in Nigeria.
Chiedozie Egesi, who led the work on biotic trait markers, explains the importance of combining varieties from South America with varieties from Africa: “Because cassava is not native to Africa, those varieties are not as genetically diverse, so we needed to bring genetic diversity from the centre of origin: South America. Coupling resistance genes from African varieties with genes for very high yields from South America was critical.”
Cassava research leaps forward with new varieties to benefit farmers
GCP’s first investment phase into cassava research stimulated a sturdy injection of people, passion, knowledge and funds into the second phase of research. From the genome maps created during the first phase, some of the world’s best geneticists would now apply genomic tools and molecular breeding approaches to increase and accelerate the genetic gains during breeding, combining farmers’ favourite characteristics with strong resistances and tolerances to abiotic and biotic constraints.
In the sprawling, tropical city of Accra on Ghana’s coast, the second phase of the research was officially launched at the end of the wet season in mid-2010. NRCRI’s Emmanuel Okogbenin coordinated product delivery from the projects, but the roles of Principal Investigator for the different projects were shared between another four individuals.
These were breeder and geneticist Chiedozie Egesi (NRCRI, Nigeria), molecular geneticist Morag Ferguson (IITA), genomic scientist Pablo Rabinowicz (University of Maryland, USA) and physiologist and geneticist Alfredo Alves (Brazilian Corporation of Agricultural Research, EMBRAPA). The team shared the vision of enabling farmers to increase cassava production for cash, well beyond subsistence levels.
Garri, or gari, a kind of granular cassava flour used to prepare a range of foods.
If the Accra launch set the stage for the next five years of cassava collaboration, a breakthrough in Nigeria at the end of 2010 set the pace, with the release of Africa’s first cassava variety developed using molecular-breeding techniques. “It was both disease-resistant and highly nutritious – a world-first,” recalls Emmanuel proudly.
Known as UMUCASS33 (or CR41-10), it took its high yield and nutritional value from its South American background, and incorporated Nigerian resistance to devastating CMD attacks thanks to marker-assisted selection. It was also resistant to several other pests and diseases. UMUCASS33 was swiftly followed by a stream of similar disease-busting varieties, released and supplied to farmers.
Never before had cassava research been granted such a boost of recognition, scientific might and organisational will. And never before had there been so much farmer consultation or so many on-farm trials.
“Cassava was an orphan crop and with the help of GCP it is becoming more prominent,” says Chiedozie. “GCP highlighted and enhanced cassava’s role as a major and reliable staple that is important to millions of poor Africans.”
Another important challenge for scientists was to develop a higher-yielding cassava for water-limited environments. The aim was to keep mapping genes for resistance to other diseases and pests and then combine them with favourable genetics that increase yield in drought conditions – no easy feat. Drought’s wicked effect on cassava is to limit the bulk of the tuber, or sometimes to stop the tuber forming altogether. Emmanuel led the work on marker-assisted recurrent selection for drought.
Hear from Chiedozie on the beneficial outcomes of GCP – in terms not only of variety releases but also of attracting further projects, prestige, and enthusiastic young breeders – in the video below (or on YouTube):
Many traits and many varieties
As closely as the cassava teams in Africa were working together, Chiedozie recalls that each country’s environment demanded different cassava characteristics: “We had to select for what worked best in each country, then continue with the research from there. What works fine for East Africa may not be so successful in Nigeria or Ghana”. A core reference set representing most of the diversity of cassava in Africa was improved with the addition of over 564 varieties. Improving the reference set, says project leader Morag Ferguson, “enables the capture of many diverse features of cassava” within a relatively small collection, providing a pathway for more efficient trait and gene discovery.
While mapping of cassava’s genetic makeup carried on, with a focus on drought tolerance, researchers continued to develop a suite of new varieties. They mapped out further genes that provided CMD resistance. In Tanzania, four new varieties were released that combined resistance to both CMD and CBSD – two for the coastal belt and two for the semi-arid areas of central Tanzania. These new varieties had the potential to double the yield of existing commercial varieties. In Ghana too, disease-resistant varieties were being developed.
Built-in disease resistance can make a huge difference to the health of cassava crops. This photo shows a cassava variety resistant to African cassava mosaic virus (ACMV), which causes cassava mosaic disease (CMD), growing on the left, alongside a susceptible variety on the right.
Meanwhile in Nigeria, another variety was released in 2012 with very high starch content – an essential factor in good cassava. This is a critical element to breeding any crop, explains Chiedozie: “A variety may be scientifically perfect, based on a researcher’s perspective, but farmers will not grow it if it fails the test in terms of taste, texture, colour or starchiness.”
Geoffrey Mkamilo, cassava research leader at Tanzania’s Agricultural Research Institute, Naliendele, says that farmer awareness and adoption go hand in hand. Once they had the awareness, he says, “the farmers were knocking on our doors for improved varieties. They and NGOs were knocking and calling.”
After groundwork in Ghana and Nigeria to find potential sources of resistance, cassava varieties that are resistant to bacterial blight and green mites were also developed in Tanzania and then tested. By the time GCP closed in December 2014, these varieties were on their way to commercial breeders for farmers to take up.
Scientists seeking to resolve the bigger challenge of drought resistance have achieved significant answers as well. Researchers have been able to map genetic regions that largely account for how well the crops deal with drought.
Hunt for ‘super powered’ cassava
The hunt was on for drought-tolerance genes in African cassava plants. The end goal was to find as many different drought-related genes as possible, then to put them all together with the applicable disease and pest resistance genes, to make a ‘super powered’ set of cassava lines. Molecular breeders call this process ‘pyramiding’, and in Ghana, Elizabeth Parkes led these projects.
With the help of Cornell University scientists, the researchers compared plants according to their starch content, how they endured a dry season, how they used sunlight and how they dealt with pests and diseases.
Fourteen gene regions or quantitative trait loci (QTLs) were identified for 10 favourable traits from the genetic material in Ghana, while nine were found for the plants in Nigeria – with two shared between the plants from both Ghana and Nigeria. After that success, the identified genes were used in breeding programmes to develop a new generation of cassava with improved productivity.
Pyramiding is important in effective disease resistance; Chiedozie explains in the video below (or on YouTube):
New cassava varieties rich in pro-vitamin A have a telltale golden hue.
The research has also delivered results in terms of Vitamin A levels in cassava. In 2011, the NRCRI team, together with IITA and HarvestPlus (another CGIAR Challenge Programme focussed on the nutritional enrichment of crops), released three cassava varieties rich in pro-vitamin A, which hold the potential to provide children under five and women of reproductive age with up to 25 percent of their daily vitamin A requirement. Since then, the team has aimed to increase this figure to 50 percent. In 2014, they released three more pro-vitamin A varieties with even higher concentrations of beta-carotene.
A field worker at IITA proudly displays a high-yielding, pro-vitamin A-rich cassava variety (right), compared with a traditional variety (left).
The new varieties developed with GCP support are worth their weight in gold, says Chiedozie: “Through these varieties, people’s livelihoods can be improved. The food people grow should be nutritious, resistant and high-yielding enough to allow them to sell some of it and make money for other things in life, such as building a house, getting a motorbike or sending their kids to school.”
Turning from Nigeria to Tanzania, Geoffrey has some remarkable numbers. He says that the national average cassava yield is 10.5 tonnes per hectare. He adds that a new cassava variety, PWANI, developed with GCP support and released in 2012, has the potential to increase yields to 51 tonnes per hectare. And they don’t stop there: the Tanzanian researchers want to produce three million cuttings and directly reach over 2,000 farmers with these new varieties, then scale up further.
A farmer tends her cassava field in northern Tanzania.
Cassava grows up: looking ahead to supporting African families
Emmanuel reflects on how the first release of a new disease-resistant high-yielding cassava variety took fundamental science towards tangible realities for the world’s farmers: “It was a great example of a practical application of marker technology for selecting important new traits, and it bodes well for the future as markers get fully integrated into cassava breeding.”
Emmanuel further believes that GCP’s Cassava Research Initiative has given research communities “a framework for international support from other investors to do research and development in modern breeding using genomic resources.” Evaluations have demonstrated that molecular-assisted breeding can slash between three and five years from the timeline of developing better crops.
Women tend to bear most of the burden of cassava cultivation and preparation. Here a Congolese woman pounds cassava leaves – eaten in many regions in addition to cassava roots – prior to cooking a meal for her family.
But, like cassava’s long growth cycle underground, Emmanuel knows there is still a long road to maturity for cassava as a crop for Africa and in research. “Breeding is just playing with genetics, but when you’re done with that, there is still a lot to do in economics and agronomics,” he says. Revolutionising cassava is about releasing improved varieties carefully buttressed by financial incentives and marketing opportunities.
Rural women in particular stand to benefit from improved varieties – they carry most of the responsibility for producing, processing and marketing cassava. So far, Elizabeth explains: “Most women reported an increase in their household income as a result of the improved cassava, but that is still dependent on extra time spent on cassava-related tasks” – a burden which she aims to diminish.
Elizabeth emphasises that future improvement research has to take a bottom-up approach, first talking to female farmers to ensure that improved crops retain characteristics they already value in addition to the new traits. “Rural families are held together by women, so if you are able to change their lot, you can make a real mark,” she says. Morag echoes this hope: “We are just starting to implement this now in Uganda; it’s a more farmer-centric approach to breeding”. The cassava teams emphasise the importance of supporting women in science too; the Tanzanians teams are aiming for a target of 40 percent women in their training courses.
Meet Elizabeth in the podcast below (or on PodOmatic), and be inspired by her passion when it comed to women in agriculture and in science:
This direct impact goes much further than individuals, says Chiedozie. “GCP’s daring has enabled many national programmes to be self-empowered, where new avenues are unfolding for enhanced collaboration at the local, national and regional level. We’re seeing a paradigm shift.” And Chiedozie’s objectives reach in a circle back to his compatriots: “Through GCP, I’ve been able to achieve my aims of using the tools of science and technology to make the lives of poor Africans better by providing them with improved crops.”
GCP has been crucial for developing the capacity of countries to keep doing this level of research, says Chiedozie: “The developing-country programmes were never taken seriously,” he says. “But when the GCP opportunity to change this came up we seized it, and now the developing-country programmes have the boldness, capacity and visibility to do this for themselves.”
Emmanuel says his proudest moment was when GCP was looking for Africans to take up leadership roles. “They felt we could change things around and set a precedent to bring people back to the continent,” he says. “They appreciated our values and the need to install African leaders on the ground in Africa rather than in Europe, Asia or the Americas.”
“If you want to work for the people, you have to walk with the people – that’s an African concept. Then when you work with the people, you really understand what they want. When you speak, they know they can trust you.” GCP trusted and trod where others had not before, Chiedozie says.
Elizabeth agrees: “In the past, the assumption was always that ‘Africa can’t do this.’ Now, people see that when given a chance to get around circumstances – as GCP has done for us through the provision of resources, motivation, encouragement and training – Africa can achieve so much!”
More links
Profiles here on the Sunset Blog: Elizabeth’s story | Chiedozie’s story
And an extra Sunset helping of cassava in the stories of our partnerships: ARI, Tanzania | CRI, Ghana | NRCRI, Nigeria
Farmer Maria Mtele holds recently harvested orange-fleshed sweetpotatoes in a field in Mwasonge, Tanzania.
Sweetpotato has a long history as a lifesaver. The Japanese used it when typhoons demolished their rice fields. It kept millions from starvation in famine-plagued China in the early 1960s and came to the rescue in Uganda in the 1990s, when a virus ravaged the cassava crop.
In sub-Saharan Africa, sweetpotato is proving crucial in the fight against blindness, disease and premature death among children under five. And, as agriculture becomes more market-oriented across the continent, sweetpotato has some significant advantages: it requires fewer inputs and less labour than other crops such as maize, tolerates marginal growing areas and can mature within four months.
On these fertile grounds, researchers across the globe are not underestimating the importance of sweetpotato as a staple crop.
“Yields achieved by resource-poor farmers in sub-Saharan Africa are typically low,” says Roland Schafleitner of the International Potato Center (CIP), based in Peru.
“Improved and well-adapted sweetpotato varieties with increased tolerance to drought, pests and diseases will have a positive impact on food and income security in sub-Saharan Africa and can significantly contribute to increasing productivity,” he says.
Roland was Principal Investigator of two research projects funded by the CGIAR Generation Challenge Programme (GCP), which developed genetic and genomic resources for breeding improved sweetpotato.
At the outset of the work, Roland says: “Breeding efforts were limited by the crop’s genetic complexity and the lack of information available about its genetic resources.
“It was clear that if we could develop genetic tools and make concerted efforts towards understanding the gene pool of sweetpotato, the breeding potential of the crop would improve.”
Farmer Mwanaidi Rhamdani at work in an orange-fleshed sweetpotato field in Mwasonge, Tanzania.
Sub-Saharan Africans getting their vitamin A from sweetpotato
Sweetpotato diversity.
Malnutrition does not always mean a simple lack of calories; research suggests that nutrient shortfalls are an even bigger killer. Vitamin A deficiency is a leading cause of blindness, infectious disease and premature death among children under five and pregnant women in sub-Saharan Africa and Asia.
Sweetpotato comes in a wide range of colours. Varieties with dark orange flesh are naturally very rich in the pigment beta-carotene, which the body converts into vitamin A. However, the sweetpotatoes traditionally grown in Africa are pale-fleshed and low in beta-carotene. African consumers were not used to eating colourful sweetpotato – and these orange-fleshed varieties were in any case not well adapted African growing conditions.
Recent years have therefore seen a collaborative effort by researchers across the world to breed orange-fleshed sweetpotato varieties fortified with high levels of beta-carotene, and even enriched with other nutrients, that have also been crossed with local varieties and so are adapted to local conditions and tastes. A crucial part of these efforts has also been to create public awareness and encourage people to grow, eat and buy these new varieties.
Two cheeky young chappies from Mozambique enjoy the sweet taste of orange-fleshed sweetpotato rich in beta-carotene, or pro-vitamin A.
All of this adds to the growing momentum behind sweetpotato. The growing awareness of sweetpotato’s potential nutritional benefits for the poor and food insecure, as well as its value for subsistence farmers as a reliable crop that withstands drought and requires minimal inputs, mean that it is growing in significance.
Orange-fleshed sweetpotato can be used to make a variety of tasty products from doughnuts to chapati.
More than 95% of the world’s sweetpotato crop is grown in developing countries, where it is the fifth most important staple food crop. It is particularly important in many African countries: Madagascar in Southern Africa; Nigeria in West Africa; and those surrounding the Great Lakes in East and Central Africa – Uganda, Malawi, Angola and Mozambique.
According to 2013 figures from the Food and Agriculture Organization of the United Nations, 3.6 million hectares of sweetpotato were harvested in Africa. While the average global yield of sweetpotato per hectare was 14.8 tonnes, across all East African countries in 2013 it was only half this, at 7.1 tonnes per hectare. In West African nations the average yield was even worse, at 3.7 tonnes per hectare.
Farmers are unable to make the most of their crops because the varieties available to them, including traditional varieties (or landraces) have low resistance to viral diseases and insect pests, and poor tolerance to drought. It is therefore crucial that when developing new varieties breeders are able to efficiently incorporate pest and disease resistance and drought tolerance traits.
New DNA markers identified for sweetpotato disease
The sweetpotato virus disease (SPVD) is the most serious disease affecting sweetpotato in sub-Saharan Africa. It often causes serious yield losses of up to 80–90 percent.
The disease is the result of joint infection by two viruses: the sweetpotato feathery mottle virus and the sweetpotato chlorotic stunt virus. Of the two, the stunt virus is the more problematic.
Wolfgang Grüneberg, also from CIP, says that, in the years 2006–2008, 52 new DNA markers were developed as part of GCP-funded research to improve marker-assisted selection for resistance to the disease.
“The results,” says Wolfgang, Principal Investigator for the research, “looked promising for developing a large number of orange-fleshed sweetpotatoes with resistance to SPVD.”
Immediately following the development of the markers, two varieties of sweetpotato were developed using a cloned gene, Resistan, known to confer resistance to the virus. The first variety was used to improve an SPVD test system so that the disease could be diagnosed earlier if a crop was affected. The second variety underwent field tests in regions in Uganda that were highly affected by the disease.
Sweetpotato vines and roots.
Mobilising the genetic diversity of sweetpotato for breeding
The goals of the GCP-supported work were to develop a diverse genetic resource base for sweetpotato and stimulate the use of new tools in ongoing breeding programmes.
To help transfer this work from high-end laboratories to resource-poor research labs in developing countries, GCP promoted collaboration across institutions and borders. Researchers from Brazil, Mozambique, Uganda and Uruguay worked together on sweetpotato genetic research projects.
As Roland explains, the basic first steps needed to begin to ‘mobilise’ the genetic diversity of sweetpotato were developing a reference set of varieties and improving genomics tools to work with polyploid crops, i.e. those possessing multiple sets of chromosomes, such as sweetpotato.
GCP-supported researchers in Peru and sub-Saharan Africa defined a reference set of 472 varieties of sweetpotato, carefully selected and honed to represent both the diversity of the crop and its most important agronomical and nutritional traits.
“Based on a reference set, genetic markers can be developed that are associated with important characteristics of the crop and can help breeders to select favourable genotypes,” says Roland.
The gene sequences developed during the Programme are now available as a Sweetpotato Gene Index.
“Based on these sequences,” says Roland, “molecular markers have been designed that can help breeders and gene-bank curators to assess the genetic diversity of their accessions and to perform genetic mapping studies.
“Today, techniques that yield a much larger number of markers for genetic studies and selection are accessible for sweetpotato,” he says.
Mwanaidi Rhamdani (left) works with Maria Mtele in an orange-fleshed sweetpotato field in rural Tanzania.
The genetic lifelines reach Africa
Sweetpotato is one of the most important staple crops in Mozambique, ranking in third position after cassava and maize. The areas harvested in Mozambique in 2013 were 1.7 million hectares of maize, 780,000 hectares of cassava and 120,000 hectares of sweetpotato.
A child eats cooked orange-fleshed sweetpotato in Uganda.
GCP funded breeders in Mozambique and Uganda to learn how to identify genetic markers that would prove useful for future sweetpotato breeding.
“Our African partners visited us at CIP and helped us complete the work on identifying markers,” recalls Roland. “This provided the opportunity for direct ‘technology transfer’ to breeders in the target region.”
The collaboration had, for the first time, created a critical amount of genetic and genomics resources for sweetpotato. The resulting Sweetpotato Gene Index and the new markers were published in a peer-reviewed journal, BMC Genomics (2010) 11:604.
The new genetic resources are in use at CIP in Peru and in breeding programmes in Burkina Faso, Mozambique, Uganda, Uruguay and the USA for the assessment of the genetic diversity of germplasm collections.
“The markers have been used for diversity analysis, especially at the CIP gene bank, and also in Africa,” says Roland, who says the markers will help future research.
“Such analysis guides germplasm conservation decisions, and diversity studies are a great tool to develop core collections and composite genotype sets – subsets of the whole collection – which allow for more practical screening for specific traits than large collections.”
“You can use any technology in the world, you can develop any product, but you need the products that farmers are willing to grow in their field.”
Chiedozie emphasises that such training opportunities are vital for the future food security of Africa. “We raised up a new crop of cassava breeders in Africa – people who were bold enough to take up a molecular breeding project and pursue it with support from the international centres. And today we are seeing the results of that. Cassava breeding programmes are standing today because of our quality of seeds sown in the past.”
