Details for Laboratory Information Management Software for genotyping workflows: applications in high throughput crop genotyping

Jayashree B, Reddy PT, Leeladevi Y, Crouch JH, Mahalakshmi V, Hutokshi, K Buhariwalla, Eshwar KE, Mace E, Folkertsma R, Senthilvel S, Varshney RK, Seetha K, Rajalakshmi R, Prasanth VP, Chandra  S, Swarupa L, Srikalyani P and Hoisington DA (2006). Laboratory Information Management Software for genotyping workflows: applications in high throughput crop genotyping. BMC Bioinformatics 7:383. (DOI: 10.1186/1471-2105-7-383).

A laboratory information management system (LIMS) has been designed and implemented at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) that meets the requirements of a moderately high throughput molecular genotyping facility. The application is designed as modules and is simple to learn and use. The application leads the user through each step of the process from starting an experiment to the storing of output data from the genotype detection step with auto-binning of alleles; thus ensuring that every DNA sample is handled in an identical manner and all the necessary data are captured. The application keeps track of DNA samples and generated data. Data entry into the system is through the use of forms for file uploads. The LIMS provides functions to trace back to the electrophoresis gel files or sample source for any genotypic data and for repeating experiments. The LIMS is being presently used for the capture of high throughput SSR (simple-sequence repeat) genotyping data from the legume (chickpea, groundnut and pigeonpea) and cereal (sorghum and millets) crops of importance in the semi-arid tropics.

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