Details for Development of cost-effective SNP assays for chickpea genome analysis and breeding

Varshney RK, Nayak S, Jayashree B, Eshwar K, Upadhyaya HD and Hoisington DA (2007). Development of cost-effective SNP assays for chickpea genome analysis and breeding. Journal of SAT Agricultural Research 3(1):29–31.

Although microsatellite or simple sequence repeat (SSR) markers have been the preferred markers for plant genetics and breeding, single nucleotide polymorphisms (SNPs) are the most common class that detect the smallest unit of genetic variation present in genomes (Rafalski 2002). Marker technologies exploiting the potential of SNPs provide the possibility of constructing genetic maps at 100-fold higher marker densities than with other types of DNA polymorphisms (Cho et al. 1999, Sachidanandam et al. 2001). Identification and mapping of SNPs has been initiated recently for crop species like rice (Oryza sativa) (Feltus et al. 2004), maize (Zea mays) (Tenaillon et al. 2001), wheat (Triticum aestivum) (Somers et al. 2003), barley (Hordeum vulgare) (Kota et al. 2001, Rostoks et al. 2005), ryegrass (Lolium multiflorum) (Miura et al. 2005) and rye (Secale cereale) (Varshney et al. 2007).