Details for An integrated pipeline of open source software adapted for multi-CPU architectures: use in the large-scale identification of single nucleotide polymorphisms

Jayashree B, Hanspal MS, Srinivasan R, Vigneshwaran R, Varshney RK, Naya S, Eshwar K, Ramesh N, Chandra S and Hoisington DA (2007). An integrated pipeline of open source software adapted for multi-CPU architectures: use in the large-scale identification of single nucleotide polymorphisms. Comparative and Functional Genomics 2007, Article ID 35604 7 pp7. (DOI: 10.1155/2007/35604).

The large amounts of EST sequence data available from a single species of an organism as well as for several species within a genus provide an easy source of identification of intra- and interspecies single nucleotide polymorphisms (SNPs). In the case of model organisms, the data available are numerous, given the degree of redundancy in the deposited EST data. There are several available bioinformatics tools that can be used to mine this data; however, using them requires a certain level of expertise: the tools have to be used sequentially with accompanying format conversion and steps like clustering and assembly of sequences become time-intensive jobs even for moderately sized datasets.